I'm looking around for a DSLR for use with a microscope.
To cut down on vibration I would like to have a mirror lock.
(I will be using x20 and x40 objectives.)
Also a remote shutter release would be an advantage.
I have read about iso settinga, and would also think of using a high
value setting - say iso 1600. I understand what noise is, but can find
little information comparing different cameras in this respect; ie which
cameras are more noisy than others.
Any suggestions as to suitable models to look at gratefully received.
Robert
John A. Stovall - 25 Nov 2005 22:54 GMT
>I'm looking around for a DSLR for use with a microscope.
>
[quoted text clipped - 9 lines]
>
>Any suggestions as to suitable models to look at gratefully received.
Take a look at the Canon 20A. Yes, A not D.
http://www.bobatkins.com/photography/digital/eos20da.html
Mirror lock up and live preview.
Canon is known for it's low noise at high ISO's.
http://www.luminous-landscape.com/reviews/cameras/20d-part2.shtml
*********************************************************
"I have been a witness, and these pictures are
my testimony. The events I have recorded should
not be forgotten and must not be repeated."
-James Nachtwey-
http://www.jamesnachtwey.com/
David Littlewood - 25 Nov 2005 23:52 GMT
>>I'm looking around for a DSLR for use with a microscope.
>>
[quoted text clipped - 19 lines]
>
>http://www.luminous-landscape.com/reviews/cameras/20d-part2.shtml
First, it's the 20Da.
Second, it costs over twice as much as the 20D.
Third, it needs to be fitted with an IR filter for most uses; it is
optimised for astrophotography and in particular the H alpha IR light.
Fourth, Canon state the live preview is only for use with very dim
images; I have not seen any information confirming that it can be used
in this application (though it would be very good to have it so
confirmed).
To the OP
You will find more information on the web if you search using the
correct term, photomicrography. SLRs of any kind (digital or film) are
not necessarily the best devices to use for PM work. However, they are
perfectly practicable.
The Canon 10D and 20D are satisfactory; they have mirror lock-up (which,
as you suggest, is essential) and they will meter quite happily when
used without a lens fitted. They use a CMOS sensor which has a very good
low noise performance. I have used my 10D with extreme ND filtration and
30 second exposures to test this, and the images are perfectly usable.
If you are on a tight budget and don't particularly need to use the DSLR
for non-PM work, then the 10D has more resolution than you will be able
to get from your microscope, and a second hand one will be half the
price of a 20D. The 20D will wildly oversample the image and waste
resolution.
The main drawback I find with a DSLR on a microscope is focussing. The
focus screens on most AF cameras are very poor for manual focussing;
even using a Canon right-angle finder with 2.5x magnification it is not
easy to get really spot-on focussing. In many ways a good compact is
easier to use and will give equally good results in most circumstances.
I use a Nikon Coolpix 995; the later 4500 may be better (4MP). This
family is very popular in the PM community, and you will find plenty of
information on the web about using them on a microscope. The video
output is very useful for focussing.
Tell us what kind of subjects you will be using it for, and what you
want to do with the output and we can maybe give more specific help.
Also, you will find far more expertise in sci.techniques.microscopy and
the Yahoo Microscopes group.
David

Signature
David Littlewood
G.T. - 26 Nov 2005 03:24 GMT
>>I'm looking around for a DSLR for use with a microscope.
>>
[quoted text clipped - 13 lines]
>
> http://www.bobatkins.com/photography/digital/eos20da.html
That's 20Da. It's a 20D, hence the D in the Da, with astrophotography
features. Christ, it shows the D in the headline of the article you
posted the URL to.
Gregh
John Passaneau - 26 Nov 2005 00:39 GMT
I use a Canon Digital Rebel for that purpose. The main drawbacks are:
No mirror lockup
A very poor viewing screen for microscope work.
Otherwise it does a good job.
I shoot at ISO 100 and see no sign of noise with exposure times up to about
2 second.
Canon makes a electronic remote release that works well but I now use some
software that came free with the camera for remote control. Its a better
method as I can change the exposure from the keyboard and other functions
too. And I get a bigger LCD display, that is full screen on the laptop if I
wish. Also I can download the pictures to the laptop as I shoot, and as I
run a wireless network, the pictures can be sent to my main computer for
processing and printing as desired, a good thing as I shoot RAW. If I was
able to do it over again I would get the 20D but I would like very much a
DSLR with interchangeable view screens so I could have one that worked
better than the one in the Digital Rebel.

Signature
John Passaneau
State College Pa.
jxp16@psu.edu
> I'm looking around for a DSLR for use with a microscope.
>
[quoted text clipped - 11 lines]
>
> Robert
Rita Ä Berkowitz - 26 Nov 2005 00:49 GMT
> I use a Canon Digital Rebel for that purpose. The main drawbacks are:
> No mirror lockup
[quoted text clipped - 29 lines]
>>
>> Robert
Kyle Jones - 26 Nov 2005 01:31 GMT
> I'm looking around for a DSLR for use with a microscope.
>
> To cut down on vibration I would like to have a mirror lock.
> (I will be using x20 and x40 objectives.)
The Olympus E-500 has mirror lockup, which is perplexingly called
"anti-shake" in the camera settings.
> Also a remote shutter release would be an advantage.
There's a wireless remote sold separately.
Rich - 26 Nov 2005 19:40 GMT
>> I'm looking around for a DSLR for use with a microscope.
>>
[quoted text clipped - 7 lines]
>
>There's a wireless remote sold separately.
It's resolution and sensor size would make it the ideal microscopy
camera.
-Rich
Volvox - 26 Nov 2005 19:09 GMT
Many thanks for useful replies - and so rapid in coming.
I should perhaps have explained that some of the objects I wish to
photograph move fast especially at x400 - vibration has been a problem
with my 35mm Dynax 5 hence my questions. Often iso 400 is not
sufficiently sensitive at x400 to record moving objects at above x100.
Yes, indeed, if it doesn't move, iso 200! That is certainly the best way
and produced good results. Fuji film particularly good for the purpose,
(but now i'm drifting off topic, so apologies to moderator!)
> I'm looking around for a DSLR for use with a microscope.
>
[quoted text clipped - 11 lines]
>
> Robert
David Littlewood - 27 Nov 2005 01:54 GMT
>Many thanks for useful replies - and so rapid in coming.
>I should perhaps have explained that some of the objects I wish to
[quoted text clipped - 5 lines]
>way and produced good results. Fuji film particularly good for the
>purpose, (but now i'm drifting off topic, so apologies to moderator!)
I did some tests with my 10D at ISO 1600 and using a crossed polariser
attenuator to give 30 second exposures. Some of the images were placed
in my folder on the Yahoo microscopes group (IIRC, it's called David L).
As far as I know they are still there.
David

Signature
David Littlewood
Rich - 27 Nov 2005 02:48 GMT
Check out this page. The company makes adapters for various cameras
plus optics to replace conventional microscope eyepieces.
http://scopetronix.com/
>Many thanks for useful replies - and so rapid in coming.
>I should perhaps have explained that some of the objects I wish to
[quoted text clipped - 21 lines]
>>
>> Robert
Pete D - 27 Nov 2005 07:25 GMT
> I'm looking around for a DSLR for use with a microscope.
>
[quoted text clipped - 11 lines]
>
> Robert
Pete D - 27 Nov 2005 07:26 GMT
Robert,
Just how much are you prepared to spend?
Cheers.
Pete
> I'm looking around for a DSLR for use with a microscope.
>
[quoted text clipped - 11 lines]
>
> Robert
Philip Procter - 30 Nov 2005 04:29 GMT
I use a Minolta 5d for microscopy in our lab. It has a built in
antishake technology on the sensor that eliminates movement both from
the camera and the microscope.
As far as I'm concerned, that single feature means more to me in the
real world than all the other stuff put together.
Minolta also has a right angle eyepiece. This is a critical accessory
if your scope is like my Olympus and the camera sits parallel to the
stage.
There is also a remote shutter release available.
I've heard the 7d has a bit less noise at high ISO than the 5d, but
I've not found the 5d's noise to be an issue. It's a very smooth
image.
Philip
>I'm looking around for a DSLR for use with a microscope.
>
[quoted text clipped - 11 lines]
>
>Robert
Philip Procter - 30 Nov 2005 04:30 GMT
I use a Minolta 5d for microscopy in our lab. It has a built in
antishake technology on the sensor that eliminates movement both from
the camera and the microscope.
As far as I'm concerned, that single feature means more to me in the
real world than all the other stuff put together.
Minolta also has a right angle eyepiece. This is a critical accessory
if your scope is like my Olympus and the camera sits parallel to the
stage.
There is also a remote shutter release available.
I've heard the 7d has a bit less noise at high ISO than the 5d, but
I've not found the 5d's noise to be an issue. It's a very smooth
image.
Philip
>I'm looking around for a DSLR for use with a microscope.
>
[quoted text clipped - 11 lines]
>
>Robert